LOCUS       sequence-72269-e         400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALE-mRuby2 against Mus spretus minor satellite sequence containing 
            two SNPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM4SpMi-01
FEATURES             Location/Qualifiers
     source          1..400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    87..198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     187..204
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(227..244)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(260..289)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
       61 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
      121 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
      181 ggcaactaga aggcacagtc gaggctgatc agcgggttta aactcaatgg tgatggtgat
      241 gatgaccggt atgcatattc agatcctctt ctgagatgag tttttgttcg aagggccctc
      301 tagactcgag gtacgccaca gcgtatgatg cgtatttttt tttttttttt tttttttttt
      361 tttttttttt tttttttttt tttttttttt tttttttttt
//
