LOCUS       sequence-72271-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding for the ER targeted and FLAGM1 
            tagged redox probe roGFPiE (roGFP C147, E147b, C204) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, 
            Melo EP, Kaminski CF, Ron D
  TITLE     Lifetime imaging of a fluorescent protein sensor reveals surprising 
            stability of ER thiol redox.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.
  PUBMED    23589496
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-04-15- 15"; volume: "201"; 
            issue: "2"; pages: "337-49"
COMMENT     Sequence Label: ERroGFP_iE_pCDNA3
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             177..890
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCESNVYIMADKQKNGIKA
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(204..232)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     830..850
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccgc catgagcgcc ctgctgatcc
      121 tggctctcgt gggcgctgcc gtggccgact acaaggacga cgacgacaag tccggaagta
      181 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      241 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      301 cccttaaatt tatttccact actggaaaac tacctgttcc atggccaaca cttgtcacta
      361 ctctgagtta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
      421 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactataagc ttcaaagatg
      481 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      541 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      601 acaactataa ctgcgagtcc aatgtataca tcatggcaga caaacaaaag aatggaatca
      661 aagcgaactt caaaaccaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      721 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      781 ccacatgctc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
      841 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa gatgagctat
      901 aaggtaccga gctcggatcc actagtaacg gccgc
//
