LOCUS       sequence-72276-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding for the ER targeted and FLAGM1 
            tagged insensitive variant of fluorescent redox probe roGFPiE with 
            Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, 
            Melo EP, Kaminski CF, Ron D
  TITLE     Lifetime imaging of a fluorescent protein sensor reveals surprising 
            stability of ER thiol redox.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.
  PUBMED    23589496
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-04-15- 15"; volume: "201"; 
            issue: "2"; pages: "337-49"
COMMENT     Sequence Label: ERroGFP_iE_C204Q_pCDNA3
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(204..232)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(944..962)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(945..962)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(982..999)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    988..1099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccgc catgagcgcc ctgctgatcc
      121 tggctctcgt gggcgctgcc gtggccgact acaaggacga cgacgacaag tccggaagta
      181 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      241 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      301 cccttaaatt tatttccact actggaaaac tacctgttcc atggccaaca cttgtcacta
      361 ctctgagtta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
      421 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactataagc ttcaaagatg
      481 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      541 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      601 acaactataa ctgcgagtcc aatgtataca tcatggcaga caaacaaaag aatggaatca
      661 aagcgaactt caaaaccaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      721 atcaacaaaa tactccaatt ggcgacggac ccgtcctgct gcctgataat cactatctga
      781 gcacacagtc cgccctgtct aaggacccca atgaaaaaag agatcatatg gtcctgctgg
      841 aatttgtcac cgccgcaggg attactcacg gaatggatga actgtataag gacgaactgt
      901 gataaagatc tggtacctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
      961 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1021 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1081 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggg
//
