LOCUS       sequence-72278-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding for the ER targeted and FLAGM1 
            tagged insensitive variant of fluorescent redox probe roGFP with 
            Cysteine 147 to Serine mutation (roGFP C147S, C204).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, 
            Melo EP, Kaminski CF, Ron D
  TITLE     Lifetime imaging of a fluorescent protein sensor reveals surprising 
            stability of ER thiol redox.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.
  PUBMED    23589496
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-04-15- 15"; volume: "201"; 
            issue: "2"; pages: "337-49"
COMMENT     Sequence Label: ERroGFP_C147S_pCDNA3
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(204..232)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     827..847
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccgc catgagcgcc ctgctgatcc
      121 tggctctcgt gggcgctgcc gtggccgact acaaggacga cgacgacaag tccggaagta
      181 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      241 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      301 cccttaaatt tatttccact actggaaaac tacctgttcc atggccaaca cttgtcacta
      361 ctctgagtta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
      421 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactataagc ttcaaggacg
      481 acgggaatta caagacacgg gccgaagtga agtttgaggg agatactctg gtgaacagga
      541 ttgaactgaa agggattgat ttcaaggagg acggaaacat cctgggccac aaactggaat
      601 acaactacaa cagtcataac gtgtacatca tggccgataa gcagaaaaac gggattaagg
      661 ctaacttcaa gacccggcac aacatcgagg acggctccgt gcagctggca gatcattacc
      721 agcagaacac cccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      781 catgctctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
      841 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaagat gagctataag
      901 gtaccgagct cggatccact a
//
