LOCUS       sequence-72282-e         374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALE-Ty1 against mouse major satellite sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM9B15
FEATURES             Location/Qualifiers
     source          1..374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(189..209)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(228..247)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(229..247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(314..336)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 attatgtgct gatacgtgac agcgacggtc cctaacgctg ccgggtgttg gctgagcgca
       61 acgatgtgcg cgtgtgtaaa cccatggccc accagtgcct cgtggtgctg cgccactgtc
      121 gaacgcacct tcggtttgat cttctcttgc tgctgctgac tgtagccgag cgtgcgtaga
      181 tccacctgca ccttcctctt tttcttagga gatctcatgg tggcggcccc tatagtgagt
      241 cgtattatta attaaggatc tgtaggaaaa agaanaaggc atgaacatgg ttagcanagg
      301 ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa
      361 ggcagccgtc cccc
//
