LOCUS       sequence-72283-e        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mam vector encoding for the ER targeted and FLAGM1 tagged 
            insensitive variant of fluorescent redox probe roGFPiE with mut 
            Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to 
            as ref GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, 
            Melo EP, Kaminski CF, Ron D
  TITLE     Lifetime imaging of a fluorescent protein sensor reveals surprising 
            stability of ER thiol redox.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.
  PUBMED    23589496
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-04-15- 15"; volume: "201"; 
            issue: "2"; pages: "337-49"
COMMENT     Sequence Label: ERrefGFP1_pCDNA3
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(204..232)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(941..959)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(942..959)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(979..996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    985..1096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccgc catgagcgcc ctgctgatcc
      121 tggctctcgt gggcgctgcc gtggccgact acaaggacga cgacgacaag tccggaagta
      181 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      241 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      301 cccttaaatt tatttccact actggaaaac tacctgttcc atggccaaca cttgtcacta
      361 ctctgagtta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
      421 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactataagc ttcaaggacg
      481 acgggaatta caagacacgg gccgaagtga agtttgaggg agatactctg gtgaacagga
      541 ttgaactgaa agggattgat ttcaaggagg acggaaacat cctgggccac aaactggaat
      601 acaactacaa cagtcataac gtgtacatca tggccgataa gcagaaaaac gggattaagg
      661 ctaacttcaa gacccggcac aacatcgagg acggctccgt gcagctggca gatcattacc
      721 agcagaacac cccaattggc gacggacccg tcctgctgcc tgataatcac tatctgagca
      781 cacagtccgc cctgtctaag gaccccaatg aaaaaagaga tcatatggtc ctgctggaat
      841 ttgtcaccgc cgcagggatt actcacggaa tggatgaact gtataaggac gaactgtgat
      901 aaagatctgg tacctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
      961 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     1021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     1081 atgaggaaat tgcatcgcat tgtctga
//
