LOCUS       sequence-72287-e        5569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Etv4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon 
            AM, Tsang M
  TITLE     Overlapping functions of Pea3 ETS transcription factors in FGF 
            signaling during zebrafish development.
  JOURNAL   Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24.
  PUBMED    20346941
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2010-06-1"; volume: "342(1)"; pages: "11-25. Epub 2010 Mar 
            24"
COMMENT     Sequence Label: Cs2+Etv4(pea3)
FEATURES             Location/Qualifiers
     source          1..5569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1618..1752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1642..1661)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1696..1715
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1865..1885)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1865..1883)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1904..1920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1904..1920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1917..1939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1928..1944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1952..1958,1959..1976,1977..1982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1997..2018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2135..2152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2306..2894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2386..2405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3065..3856,3857..3925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3688..3707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3926..4030)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4056..4511)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4193..4214)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4405..4424
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4585..5569
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agatggatta
      121 taagatggat ggatatctgg accagcaagt gccttatact ttagctaata ggtcgcaagg
      181 aaatgggccc ctaaatagac tgttgatggc gacaaaaagg aaatacatgg acgcagaatt
      241 accccctcag gaatctgaag acctctttca ggatttaagc caacttcagg agacctggct
      301 caccgaagct caagttcctg acagcgatga gcagtttgtt cctgactttc actcagagaa
      361 ctcagtggca ttccacagcc cgcctgtgaa gattaagaaa gagccgcaga gtcccggatc
      421 agaccccagc cagtcctgca gccacaagca aagcttcagc taccccaatg gagagcagtg
      481 cctttacgcc agtgcctatg agcagaagag agcagccgtg gcaggagcag gaggatctaa
      541 gagctcatgt ccagggacgc ccatgtcccc tatgcagcat tattccccca aaccaacagt
      601 gggaactcgg caggaatcag ggtatatgaa tccaccctca gccagccaat cccacgcctg
      661 ccacagccac agttacccca tgaaccccag ttccaggttc ccttccggct cggcagagat
      721 gtgcccacca ttcgcttccc aaggccaggc cctgcagcgc atgcatcctg cccatgccag
      781 cggaggcggt taccatcggc aacactctga cccctgcttg ccttatcctc ctcagcagac
      841 ctttaagcaa gagtacatgg accctctgta tgaccgagcg gcccacatta acggaccaca
      901 gcctcagagg tttcctcctg cccatatgat ggtcaaacag gagcccaccg actacactta
      961 cgaacctgat gtgcctggct gcccatccat gtaccatcac aacgaaggct actccaaccc
     1021 acagcacaac agtgaaggct acatgtttga aaatgattcc cgtgttgtgc cagagaaatt
     1081 tgaaggtgag gtgaaacaag aagggggcag tgtgtttcgt gaaggtgccc cctatcagcg
     1141 tcgtggctca cttcaactat ggcagttctt ggttgccctt cttgatgacc ccagcaatgc
     1201 ccacttcatc gcatggacgg gccgtggcat ggaattcaaa ctcattgagc cagaggaggt
     1261 ggcaagactc tgggggatgc agaagaaccg tccagccatg aactatgaca aactgagtcg
     1321 ctctttgcgt tactattatg agaagggaat tatgcaaaag gtggctggtg agcgttatgt
     1381 ttacaaattt gtgtgtgagc cagaagcttt gataaccttg gcttttcccg acaatcagcg
     1441 gccaagtctg aaggcggaat ttgagcgcta cgtcaacgag gaggacactg tgccactgtc
     1501 ccacctcgat gagggagttt cttaccctcc cgaaccagct gccaccaaca tgggccctca
     1561 gccctactcc aaaggctaca tgtactaatc tagaactata gtgagtcgta ttacgtagat
     1621 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1681 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1741 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1801 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
     1861 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     1921 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1981 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2041 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3361 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3661 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4021 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     4081 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4141 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4201 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4261 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4321 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4381 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4441 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4501 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4561 ggcctcttcg ctattacgcc agtcgaccat agccaattca atatggcgta tatggactca
     4621 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
     4681 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
     4741 tgccaattca atatggcgga cttggcactg tgccaactgg ggaggggtct acttggcacg
     4801 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
     4861 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
     4921 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
     4981 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
     5041 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
     5101 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
     5161 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
     5221 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
     5281 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
     5341 tctattaata gtaacttggc aagtacatta ctattggaag gacgccaggg tacattggca
     5401 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
     5461 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
     5521 taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaac
//
