LOCUS       sequence-72290-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Histone H2B fused with tandem iRFP, ires-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pCAG-H2BtdiRFP-IP
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..413
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      426..435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccccaggt acttgggaat tcagatctcg ccaccatgcc agagccagcg aagtctgctc
       61 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
      121 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
      181 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
      241 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
      301 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
      361 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac
      421 cggtcgccac catggccgaa gggagcgtgg caagacagcc cgatctgctg acatgcgacg
      481 acgaaccaat ccacattcca ggagctatcc agcctcatgg actgctgctg gctctggccg
      541 ctgacatgac tatcgtggca ggctctgata acctgccaga gctgaccggg ctggctatcg
      601 gagcactgat tggccggtcc gcagccgacg tgttcgattc tgagactcac aatagactga
      661 ccatcgccct ggctgaacct ggggctgcag tgggagcccc aattaccgtc ggcttcacaa
      721 tgcgaaagga cgctggcttt atcgggtcct ggcaccgcca tgatcagctg atttttctgg
      781 agctggaacc acctcagaga gacgtggcag agccacaggc tttcttt
//
