LOCUS       sequence-72294-e        5916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Etv5 T139D mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon 
            AM, Tsang M
  TITLE     Overlapping functions of Pea3 ETS transcription factors in FGF 
            signaling during zebrafish development.
  JOURNAL   Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24.
  PUBMED    20346941
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2010-06-1"; volume: "342(1)"; pages: "11-25. Epub 2010 Mar 
            24"
COMMENT     Sequence Label: CS2+MYC-Etv5:T139D
FEATURES             Location/Qualifiers
     source          1..5916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1965..2099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1989..2008)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2043..2062
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2212..2232)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2212..2230)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2251..2267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2251..2267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2264..2286)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2275..2291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2299..2305,2306..2323,2324..2329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2344..2365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2482..2499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2653..3241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2733..2752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3412..4203,4204..4272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4035..4054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4273..4377)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4403..4858)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4561)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4752..4771
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4932..5916
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctctcgaga tggacggatt ttatgaccag caagttccat ttatggtccc acctaataag
      421 tcattgcaag tggaggaacc atataacaga ccagttaatg acagaaagag aaagttagtg
      481 gagactgaac ttgctcagga cacagaggaa cttttccagg acctcagcca gctgcaggag
      541 atctggattg cagaagctca ggtgcccgat gacgaacagt ttgtcccaga tttccagtcg
      601 gagagcttga tgtttcatgg cccacccctg gccaagatca aacgggaact gagtccctcc
      661 aaagagctct ccccctgtag ccaggataga agtcccatgc cgtatggaga gaagtgcctt
      721 tacagctaca gtgcctatga acggaagccc agcactgggt tcaagccatt gactcctccc
      781 tcggaccccg tctccccatg tgtccccagc aatgccctgg cgacgcgtcc cctgcacgaa
      841 cagactccac cacctgtgtc caacacagca ctggggcagc gtctccttca tggacagcct
      901 acaatgacca agagcacacc caaccagacg tgtgtccctc accacagcca gagcccaccc
      961 tttgtagtgc cctgtccatc cctgaaccgt gaaactcact tcagtaacga acctaggttt
     1021 cagcggcaac tttcagagcc ttgccttcca ttcccacctt ctgatgccca gggaagaccc
     1081 agctttatgc cacaggtcgc ctgcacttca ccaagagatg gaagaccacc gtaccatcgc
     1141 caaatgtctg agcccctggt tcctgtacct cctcagggct tcaaacagga gctcctcgac
     1201 ccccgctatg ttgagcaggg tgttcccaac atgggcccat cccaggctgc cttccacccg
     1261 atggccatca agcaagagcc acgggacttc tgcttcgact ctgaagtgcc taaatgtcag
     1321 tcttcatttg ggagagcagc aagcttctac caaaaccatg agaacttctc atttgacaga
     1381 gaacagcagt tgtattttga tgacacctgt gtggttccag agagacttga agggaaggta
     1441 aagcaagagc cctctgtgta tcgtgaaggt ccaccatatc agcggcgcgg atctctccag
     1501 ctgtggcagt ttctggtgac gctattggat gatccttcca acggccattt cattgcctgg
     1561 actggacgtg gaatggagtt caaacttatt gagccagagg aggtggctcg acgctggggc
     1621 atccagaaga acagacccgc catgaactat gacaagctga gtcgctcact gcgctactac
     1681 tatgagaagg gaatcatgca gaaggttgcc ggcgagaggt acgtctacaa atttgtgtgc
     1741 gatcctgaag cgctcttctc catggccttc ccagacaacc agaggcccaa tctcaaagca
     1801 gaccctgacg gcctgcccac tgtggatgat gacacacttc cattggcaca ttatgacgac
     1861 gggtcctcgt atttagttga cggtggcgag cagtgtgttt ctgggatgcc tttccctgat
     1921 ggttacgtgt actgatctag aactatagtg agtcgtatta cgtagatcca gacatgataa
     1981 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2041 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2101 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2161 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
     2221 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4381 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4441 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4501 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4561 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4621 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4681 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4741 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4801 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4861 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4921 ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata
     4981 tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata
     5041 tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata
     5101 tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga
     5161 ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg
     5221 cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc
     5281 caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca
     5341 tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata
     5401 tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat
     5461 atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca
     5521 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc
     5581 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc
     5641 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta
     5701 acttggcaag tacattacta ttggaaggac gccagggtac attggcagta ctcccattga
     5761 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg
     5821 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa
     5881 tgggaggtct atataagcaa tgctcgttta gggaac
//
