LOCUS       sequence-72314-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal 
            alpha-importin nuclear localization sequence. Synapsin promoter for 
            neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: LITE2.0
            pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-V...
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             431..478
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
       61 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
      121 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
      181 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
      241 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
      301 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
      361 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc
      421 cgccaccatg aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagaa
      481 gatggacaaa aagacgattg tgtggtttag acgtgacctt cgcattgagg acaaccctgc
      541 gttggcagcg gcagcgcatg aaggttcggt attccctgtc tttatctggt gtcccgaaga
      601 ggaggggcaa ttctatcccg gtcgcgcgtc ccgatggtgg atgaaacagt cgctcgcaca
      661 tcttagccag tcgttgaagg cgctcggctc tgatctgacg ctcatcaaaa cacacaatac
      721 aatttctgcc attctggatt gcattagggt gactggtgcc accaaagttg tgttcaatca
      781 cctttatgat cccgtaagcc tggtcaggga tcatacagta aaagagaaac ttgtagagag
      841 gggaatcagc gtccaatcgt acaatgggga cttgttgtat gaaccgtggg aaatctattg
      901 tgagaaaggc aaacctttca cttccttcaa ttcgtactgg aagaaatgcc tcgacatgtc
      961 catcgagtca gtcatgctgc cgccaccttg g
//
