LOCUS       sequence-72322-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with HD half repeat; fused to nuclear 
            localization sequence linker and SID4X transcriptional repressor 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: 
            pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..79)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(772..798)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctccgttc caggtagtca gcggcctcca gcagcatctg gatgttcatg ctggcctcca
       61 cctttctctt cttcttgggg ctgctagctg ccactcgatg tgatgtcctc tcgggaatcc
      121 ttctgttggt ccgcttaatc aatgcaggcg cgtgcgggag ccccttcttc accgcatcca
      181 aggcgggtcg tccgccgagg caggccagcg ctacaagatg atcattagtg agtgcggcca
      241 gcgcggggtc cggcctcgaa agctgggcca cgattgactc cagtgcgggt ctgcccccgt
      301 catgggatgc aatagccact acctgctcag gcgtgagtcg agacccgtac gggtctctgt
      361 caggttgagg ggtgctcccg tgagcgcatt gcgccacgcg tgcaccgcct cgaccgccgt
      421 gactcctccc cgcttcgcga tcttcagcaa ctggcccgtg tccagctgaa ggggaggccc
      481 tctcagctct cccgcgaccg tcaacagggc ctcaagcgct cgggctccgc tccactgttt
      541 ccccacaccg acgatcgcct catgtgtggc ttccggcaac gccgcaatca tgtcctggta
      601 cttgacggcg accgtgccaa gggctgcagg gtgctgcgac aaggctacga tgtgggcatg
      661 tgtaaaccca tgacccacca gcgcttcgtg atgctgcgcg actgtcgacc tcactttggg
      721 cttgatcttt tcctgctgct gctgtgaata tcccaaagtt ctcaaatcta cagcgtaatc
      781 tggaacatcg tatgggtaca tggtggcgga tccttctcga ctgcgctctc aggcacgaca
      841 cgactcctcc gctgcccacc gcagactgag gcagcgctga gtcgccggcg ccgcagcgca
      901 gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg gtgcggtccg gctgggccgg
      961 cggcggcgcg
//
