LOCUS       sequence-72332-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Klezovitch O, Risk M, Coleman I, Lucas JM, Null M, True LD, Nelson 
            PS, Vasioukhin V
  TITLE     A causal role for ERG in neoplastic transformation of prostate 
            epithelium.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):2105-10.
  PUBMED    18245377
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 12. 105(6):2105-10."
COMMENT     Sequence Label: pMAX-DEST
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..158
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(199..504)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(301..320)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gaactcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa agctgaacga
       61 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      121 acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg
      181 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
      241 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
      301 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
      361 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
      421 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
      481 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
      541 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
      601 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
      661 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
      721 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
      781 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca
      841 gatctttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      901 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg gcatcagcac
      961 cttgtcgcct tgcgtataat atttgcc
//
