LOCUS       sequence-72338-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with NI half repeat; fused to nuclear 
            localization sequence linker and VP64 transcriptional activator 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(105..254)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(279..299)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(989..1015)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg
      121 aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa
      181 ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa
      241 tcgtccaatg cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg
      301 ctagctgcca ctcgatgtga tgtcctctcg ggaatccttc tgttggtccg cttaatcaat
      361 gcaggcgcgt gcgggagccc cttcttcacc gcatccaagg cgggtcgtcc gccgaggcag
      421 gccagcgcta caagatgatc attagtgagt gcggccagcg cggggtccgg cctcgaaagc
      481 tgggccacga ttgactccag tgcgggtctg cccccgatat tggatgcaat agccactacc
      541 tgctcaggcg tgagtcgaga cccgtacggg tctctgtcag gttgaggggt gctcccgtga
      601 gcgcattgcg ccacgcgtgc accgcctcga ccgccgtgac tcctccccgc ttcgcgatct
      661 tcagcaactg gcccgtgtcc agctgaaggg gaggccctct cagctctccc gcgaccgtca
      721 acagggcctc aagcgctcgg gctccgctcc actgtttccc cacaccgacg atcgcctcat
      781 gtgtggcttc cggcaacgcc gcaatcatgt cctggtactt gacggcgacc gtgccaaggg
      841 ctgcagggtg ctgcgacaag gctacgatgt gggcatgtgt aaacccatga cccaccagcg
      901 cttcgtgatg ctgcgcgact gtcgacctca ctttgggctt gatcttttcc tgctgctgct
      961 gtgaatatcc caaagttctc aaatctacag cgtaatctgg aacatcgtat gggtacatgg
     1021 tggcggatcc ttctcgactg cgctctcagg cacgacacga ctcctccgct gcccaccgca
//
