LOCUS       sequence-72341-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with NG half repeat; fused to nuclear 
            localization sequence linker and VP64 transcriptional activator 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..81)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(103..252)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(277..297)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(987..1013)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcactgg gccaggattc tcctcgacgt caccgcatgt
       61 tagcagactt cctctgccct ctccactgcc tctagagtta atcagcatgt ccaggtcgaa
      121 atcatcaagg gcgtcactgc cgagcatgtc gaggtcaaag tcatcaaggg catccgaacc
      181 aagcatgtca aggtcaaaat catcgagggc gtcacttccc agcatatcca gatcaaaatc
      241 gtccaatgcg tcagcccgtc cggaaccgct ggcctccacc tttctcttct tcttggggct
      301 agctgccact cgatgtgatg tcctctcggg aatccttctg ttggtccgct taatcaatgc
      361 aggcgcgtgc gggagcccct tcttcaccgc atccaaggcg ggtcgtccgc cgaggcaggc
      421 cagcgctaca agatgatcat tagtgagtgc ggccagcgcg gggtccggcc tcgaaagctg
      481 ggccacgatt gactccagtg cgggtctgcc cccgccattg gatgcaatag ccactacctg
      541 ctcaggcgtg agtcgagacc cgtacgggtc tctgtcaggt tgaggggtgc tcccgtgagc
      601 gcattgcgcc acgcgtgcac cgcctcgacc gccgtgactc ctccccgctt cgcgatcttc
      661 agcaactggc ccgtgtccag ctgaagggga ggccctctca gctctcccgc gaccgtcaac
      721 agggcctcaa gcgctcgggc tccgctccac tgtttcccca caccgacgat cgcctcatgt
      781 gtggcttccg gcaacgccgc aatcatgtcc tggtacttga cggcgaccgt gccaagggct
      841 gcagggtgct gcgacaaggc tacgatgtgg gcatgtgtaa acccatgacc caccagcgct
      901 tcgtgatgct gcgcgactgt cgacctcact ttgggcttga tcttttcctg ctgctgctgt
      961 gaatatccca aagttctcaa atctacagcg taatctggaa catcgtatgg gtacatggtg
     1021 gcggatcctt ctcgactgcg ctctcaggca cga
//
