LOCUS       sequence-72342-e         478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALE-mRuby2 against Mus spretus/musculus minor satellite sequence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM4SpMi-05
FEATURES             Location/Qualifiers
     source          1..478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(186..206)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(225..244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(226..244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(311..333)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 atgtgctgat acgtgacagc gacggtccct aacgctgccg ggtgttggct gagcgcaacg
       61 atgtgcgcgt gtgtaaaccc atggcccacc agtgcctcgt ggtgctgcgc cactgtcgaa
      121 cgcaccttcg gtttgatctt ctcttgctgc tgctgactgt agccgagcgt gcgtagatcc
      181 acctgcacct tcctcttttt cttaggagat ctcatggtgg cggcccctat agtgagtcgt
      241 attattaatt aaggatctgt aggaaaaaga agaaggcatg aacatggtta gcagaggctc
      301 tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc ccccgaaggc
      361 agccgtcccc ccgcggacag ccccgaggct ggagagggag aaggggacgg cggcgcggcg
      421 acgcacgaag gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgcccc
//
