LOCUS       sequence-72350-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated 
            CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin 
            promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: 
            LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(533..553)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgcgccct cacgtgaatg taatccgttt tctccagttc ttttgtgact ttactgctgt
       61 cattgctgaa attgttctct tctttcttag ctttgtgctt catctttttg atacttttgg
      121 tagacccatt gttctgttca gtaatcttcg acttttcctc ctctccctcc tctaccagat
      181 catcgcgatt catggtcatt ttcttcttct tttcgttgca atcttttgtc tccgtgtcga
      241 atttccgttt cttgaagttc cctgttccca gcgtcgtttc aggcgaaatc gagagtcgcg
      301 agtcgccttc tacagtggtt tctccataca tcatcggaag attcaatccg gccgttgaga
      361 ggtagctgtc catttcccct cccgtaatca tagatgagtc cgcaaagaac ccggccaacg
      421 gggagtcgaa ggtggggtta agatacttca aatgtgcgcg ctgacgttcc aacacggaca
      481 tgtcgggaaa gttaagcagc aaatccccac caatcgcgcc gttgctggcc tccacctttc
      541 tcttcttctt ggggctgcta gctgccactc gatgtgatgt cctctcggga atccttctgt
      601 tggtccgctt aatcaatgca ggcgcgtgcg ggagcccctt cttcaccgca tccaaggcgg
      661 gtcgtccgcc gaggcaggcc agcgctacaa gatgatcatt agtgagtgcg gccagcgcgg
      721 ggtccggcct cgaaagctgg gccacgattg actccagtgc gggtctgccc ccgtcatggg
      781 atgcaatagc cactacctgc tcaggcgtga gtccgtgcgc ttggcacagc accggcagca
      841 atcgttgtac ggtttccaac gcctgtttgc cccctccatt cgaggcaatg gccacgacct
      901 gctctggggt taggccgtgc gcttggcaca gcacaggcaa cagcctctgt actgtttcca
      961 aggcttgctt tcctccgtcg tggcttgcga tcgccacgac ctgctctggg gttagcccgt
     1021 gcgcttggca cagca
//
