LOCUS       sequence-72358-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to drive the sgRNA expression under RNase P non-coding RNA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Chen C, Fenk LA, de Bono M
  TITLE     Efficient genome editing in Caenorhabditis elegans by 
            CRISPR-targeted homologous recombination.
  JOURNAL   Nucleic Acids Res. 2013 Sep 5.
  PUBMED    24013562
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Sep 5."
COMMENT     Sequence Label: sgRNA with rpr-1 promoter
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..52
                     /gene="mutant version of attB"
                     /label=attB6
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        525..600
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgatttcggc taaaaaatag cgaaaaaaca tcaaatttgt attaaaaaga agcagttgaa
       61 attttgagtg aggctcagag actacaaact acaaaaaggc tcagcctcaa ccaattttta
      121 gtgttaaaaa tttgattttt tcagttaaaa acgatggttt ttgatgcttt ttacctattt
      181 tacagataga aaattttaaa tttcgcaaaa atctttaaaa ataaactttt ttgtttggtt
      241 ttcgctcgaa aatagcttta aatttgcagt ttttctctca aaaactctaa atttcatgcg
      301 ttccacgtgg ctttctatca atttaaatac taatattaat attttcttca aaatctgcca
      361 cgttacagcc tgccatagcc ttctgaaact ctacaattcc cctctaatca ctagcgccgc
      421 ctccccgcac actcaacaca gcgagcggcg gaacccgcgg ggtgtcggcc gctcggacac
      481 gctcccgtat ataacgacgc ggcgcgcgtc aagttgtgga attcgtttta gagctagaaa
      541 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      601 ttttgtgggc ttcaggcttt ctgttgggcc gttttaggcc aaatgataga aagccacgtg
      661 gaaatcatga atttaaagct attttcgagc gaaaatca
//
