LOCUS       sequence-72359-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to drive the sgRNA expression under a U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Chen C, Fenk LA, de Bono M
  TITLE     Efficient genome editing in Caenorhabditis elegans by 
            CRISPR-targeted homologous recombination.
  JOURNAL   Nucleic Acids Res. 2013 Sep 5.
  PUBMED    24013562
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Sep 5."
COMMENT     Sequence Label: sgRNA with U6 promoter
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        335..410
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(739..756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacgtgtctt gtccaggcgc gccacccaaa tgtttttata atttggcaaa ttaccaaaaa
       61 tgtgactgga agcattaaca aaattttgaa caatattcga ggtttcaaaa tcatgatatt
      121 ttagatattt tggactgttt tgagtcaaat tgttaatgac agcattttaa gagctcccaa
      181 cacatagtgt ttccaatgtt atacccaatc aataatagca agtcaataaa ctacctctac
      241 actatttctg gtaataggcg aaacctctac actgtcagtc actttgtaag gtgtgcctat
      301 gtatttccat aatatttcat acaaattgaa gcttgtttta gagctagaaa tagcaagtta
      361 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgac
      421 taatttctgg tattattgtt tagaattgta agaaaatttt acattccaga aaatgtatcc
      481 aaaaaactta cgcaaactcg gagcgtatag ttgcatgaag tgctggatcc gaaatacgaa
      541 cgacaaattg ttgatacttt gctttcaaac tctcgtaata aggcatcaca aagttctcaa
      601 ttttattaat taatggagca caagactggc ctcatgggcc ttccgctcac tgcccgcttt
      661 ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta
      721 ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc
      781 tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      841 ctggcgtttt tccataggct ccgcccccct gacgagcatc a
//
