LOCUS       sequence-7237-ex        8319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM
  TITLE     Development of a self-inactivating lentivirus vector.
  JOURNAL   J Virol. 1998 Oct . 72(10):8150-7.
  PUBMED    9733856
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Oct . 72(10):8150-7."
COMMENT     Sequence Label: pLS-CG
FEATURES             Location/Qualifiers
     source          1..8319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             234..867
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    911..1036
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1529..1762
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1947..1991
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2315..2618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2619..2822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2773..2793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2861..2870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2867..2869,2870..2872,2873..3583)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2867..2869,2870..2872,2873..3583)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2912..2933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3173..3192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3520..3541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4120..4300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4326..4343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4332..4556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4602..5030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4689..4708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4899..4920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5039..5059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5044..5374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5225..5360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5287..5306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5422..5469
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5488..5862
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5992..6113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6029..6048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6083..6102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6162..6178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6162..6178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6175..6197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6186..6202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6210..6216,6217..6234,6235..6240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6255..6276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6393..6410)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6564..7152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6644..6663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7323..8114,8115..8183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7946..7965
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8184..8288)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttggaagg
      241 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
      301 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
      361 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
      421 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
      481 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
      541 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
      601 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
      661 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      721 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      781 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      841 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacctga aagcgaaagg
      901 gaaaccagag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      961 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1021 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1081 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1141 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1201 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1261 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1321 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1381 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1441 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1501 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1561 caggaagcac tatgggcgca gcctcaatga cgctgacggt acaggccaga caattattgt
     1621 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1681 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1741 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1801 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatc tggaatcaca
     1861 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1921 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1981 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2041 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2101 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2161 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2221 ttcgattagt gaacggatcc cccgggctgc aggaattcga ttaatagtaa tcaattacgg
     2281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2821 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
     2881 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2941 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3001 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3061 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3121 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3181 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3241 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3301 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3361 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3421 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3481 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3541 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatccta
     3601 ctagtaggat ctcgagacct ggaaaaacat ggagcaatca caagtagcaa cacagcagct
     3661 accaatgctg cttgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     3721 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     3841 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     3901 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     3961 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     4021 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     4081 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     4141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4261 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     4321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4561 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4741 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
     4801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
     4981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5041 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca
     5101 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5161 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5221 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5281 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5341 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5401 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     5461 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     5521 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     5581 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     5641 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     5701 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     5761 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     5821 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     5881 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5941 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6121 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6181 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6241 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6301 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6361 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6421 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6481 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6541 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6601 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6661 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6721 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     6781 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6841 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6901 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6961 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7021 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7081 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7141 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7201 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7261 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7321 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7381 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7441 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7501 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7561 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7621 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7681 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7741 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7801 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7861 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7921 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7981 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8041 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8101 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8161 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8221 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8281 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
