LOCUS       sequence-72372-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the alcA promoter as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGA008
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(344..366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(560..579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     679..701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(739..757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaacctcg ggatagttcc
       61 gacctaggat tggatgcatg cggaaccgca cgagggcggg gcggaaattg acacaccact
      121 cctctccacg caccgttcaa gaggtacgcg tatagagccg tatagagcag agacggagca
      181 ctttctggta ctgtccgcac gggatgtccg cacggagagc cacaaacgag cggggccccg
      241 tacgtgctct cctaccccag gatcgcatcc ccgcatagct gaacatctat ataaaacatg
      301 agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta caacgtcgtg
      361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      481 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      541 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      601 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      661 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      721 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatga
//
