LOCUS       sequence-72373-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the signal peptide for 
            protein secretion of the Bacillus megaterium protein Pac under 
            control of the xylose inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPPac-hp
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(198..215)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
      181 aattcttgga ccggttagtg atggtgatgg tgatgcgatc ctctcaggcc ggcatgcggc
      241 cggtaccgga tcccgggagc tccggagatc ttcgaactag tcccagcaaa aactagattt
      301 tgaggaaaaa tgaaaacaaa taggattatg actgatatta gccacttcgt cttcattgta
      361 cattcacctc cttgatttaa gtgaacaagt ttatccatca actatcttaa ttgagttagt
      421 ttgtttatcc aataaactaa ctttatctca tcatatatta attaaatgtt tatgtcaatg
      481 ttttttttag aaaatttagt tataatatta gatatgatac ttttaaatat ctaattcaag
      541 cttcaaaaaa caccaactta gttcggtgga taaacaaagg agtggttatt attcaaattg
      601 cagatcaagc tttagtaaaa aaaatgaatc aaaaattaat attagatgaa attttgaaga
      661 actcccctgt ctccagggca actctctctg agattacagg attaaacaag tctactgtct
      721 cctctcaagt aaatacactg cttgaaaaag attttatttt tgaaattggg gcagggcaat
      781 ctagaggcgg cagaagacct gtaatgcttg tttttaataa gaatgcaggc tactcgattg
      841 gtattgatat aggagtcgac tatcttaacg gaattctaac cgacttagaa ggaaatatta
      901 ttctcgaga
//
