LOCUS       sequence-72376-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the omega element as GreenGate N-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGB002
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    59..114
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(136..155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(137..155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(165..187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(381..400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     500..522
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(560..578)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        646..750
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaaacata tttttacaac
       61 aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa ttacaggctt
      121 gagaccgaat tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt
      181 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      241 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      301 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      361 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      481 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      541 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      601 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      721 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      781 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      841 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      901 agcgg
//
