LOCUS       sequence-72379-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter for RNA 
            polymerase of the phage SP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPSP6
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(362..380)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(363..380)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
       61 aacgcggccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
      121 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattaattag ctccaattct
      181 tggaccggtg aatccgttcg cgaggtttag ccggcatgcg gccggtaccg gatcccggga
      241 gctccggaga tcttcgaact agtttggacc attgtacatt tcacctcctt ttactatcca
      301 gccctccccg tgaagactgg tgagtaatag gccgctcaaa agaacggcac tatacttcta
      361 ttctatagtg tcacctaaat cgtatgctta aggaacgtac agacggctta aaagccttta
      421 aaaacgtttt taaggggttt gtagacaagg taaaggataa aacagcacaa ttccaagaaa
      481 aacacgattt agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa
      541 aagaacgaag tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg
      601 tctttttttg atggttttga acttgttctt tcttatcttg atacatatag aaataacgtc
      661 atttttattt tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta
      721 ttgaaaaccc ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc
      781 aaacaaataa ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt
      841 tattcagatg aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca
      901 tggagagaaa agaaaatcgc taatgttgat tactttgaac ttctgcata
//
