LOCUS       sequence-7238-ex        3909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J,
            Magendantz M, Jacks T
  TITLE     The Nf2 tumor suppressor regulates cell-cell adhesion during tissue 
            fusion.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6.
  PUBMED    17360635
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3261-6."
COMMENT     Sequence Label: pMB80 (R26-CreER)
FEATURES             Location/Qualifiers
     source          1..3909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..1110
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1123..2058
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1171..2064
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    2125..2158
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2185..2684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2220..2243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2617..2636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2724..3323
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2724..2743)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3220..3240
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    3870..3903
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctagaaagg gcgcagtagt ccagggtttc cttgatgatg tcatacttat cctgtccctt
       61 ttttttccac agccgcggac catgtccaat ttactgaccg tacaccaaaa tttgcctgca
      121 ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat
      181 cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg
      241 gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat
      301 tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc
      361 cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt
      421 tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag
      481 gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat
      541 cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt
      601 atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg
      661 ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt
      721 agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat
      781 ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc
      841 agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac
      901 ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
      961 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
     1021 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
     1081 gcaatggtgc gcctgctgga agatggcgat ctcgagccat ctgctggaga catgagagct
     1141 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
     1201 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
     1261 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
     1321 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
     1381 gatttgaccc tccatgatca ggtccacctt cttgaatgtg cctggctaga gatcctgatg
     1441 attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg
     1501 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
     1561 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
     1621 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
     1681 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
     1741 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
     1801 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
     1861 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc
     1921 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
     1981 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
     2041 gagggtttcc ctgccacagc ttgaacgcgt ggcaataaaa agacagaata aaacgcacgg
     2101 gtgttgggtc gtttgttcgc tagcataact tcgtataatg tatgctatac gaagttatga
     2161 attcgtcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     2221 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2281 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2341 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2401 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     2461 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2521 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     2581 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     2641 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag
     2701 atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     2761 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     2821 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     2881 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     2941 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     3001 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     3061 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     3121 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     3181 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     3241 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     3301 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     3361 gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt gatgatctat
     3421 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
     3481 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
     3541 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
     3601 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
     3661 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
     3721 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
     3781 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
     3841 aattccatca gaagctggtc gactctagca taacttcgta taatgtatgc tatacgaagt
     3901 tattctaga
//
