LOCUS       sequence-72383-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the signal peptide for 
            protein secretion of the Bacillus megaterium protein YngK under 
            control of the xylose inducible promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPYngK-hp
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(197..214)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
      181 attcttggac cggttagtga tggtgatggt gatgcgatcc tctcaggccg gcatgcggcc
      241 ggtaccggat cccgggagct ccggagatct tcgaactagt atctgcttta gcaggtaacg
      301 cgctcgaaca aaacagcagt aaaaacaaga tgcttcctat acatttttta atatacattg
      361 tacattcacc tccttgattt aagtgaacaa gtttatccat caactatctt aattgagtta
      421 gtttgtttat ccaataaact aactttatct catcatatat taattaaatg tttatgtcaa
      481 tgtttttttt agaaaattta gttataatat tagatatgat acttttaaat atctaattca
      541 agcttcaaaa aacaccaact tagttcggtg gataaacaaa ggagtggtta ttattcaaat
      601 tgcagatcaa gctttagtaa aaaaaatgaa tcaaaaatta atattagatg aaattttgaa
      661 gaactcccct gtctccaggg caactctctc tgagattaca ggattaaaca agtctactgt
      721 ctcctctcaa gtaaatacac tgcttgaaaa agattttatt tttgaaattg gggcagggca
      781 atctagaggc ggcagaagac ctgtaatgct tgtttttaat aagaatgcag gctactcgat
      841 tggtattgat ataggagtcg actatcttaa cggaattcta accgacttag aaggaaatat
      901 tattctcgag a
//
