LOCUS       sequence-72384-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the signal peptide for 
            protein secretion of the Bacillus megaterium protein Vpr under 
            control of the xylose inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPVpr-hp
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(198..215)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
      181 aattcttgga ccggttagtg atggtgatgg tgatgcgatc ctctcaggcc ggcatgcggc
      241 cggtaccgga tcccgggagc tccggagatc ttcgaactag tgttggcgga tgctcccatt
      301 gcaaaagaag ataaagcaac cgaaaatgct actgtactta atacagaccg aattgatact
      361 tttttcattg tacattcacc tccttgattt aagtgaacaa gtttatccat caactatctt
      421 aattgagtta gtttgtttat ccaataaact aactttatct catcatatat taattaaatg
      481 tttatgtcaa tgtttttttt agaaaattta gttataatat tagatatgat acttttaaat
      541 atctaattca agcttcaaaa aacaccaact tagttcggtg gataaacaaa ggagtggtta
      601 ttattcaaat tgcagatcaa gctttagtaa aaaaaatgaa tcaaaaatta atattagatg
      661 aaattttgaa gaactcccct gtctccaggg caactctctc tgagattaca ggattaaaca
      721 agtctactgt ctcctctcaa gtaaatacac tgcttgaaaa agattttatt tttgaaattg
      781 gggcagggca atctagaggc ggcagaagac ctgtaatgct tgtttttaat aagaatgcag
      841 gctactcgat tggtattgat ataggagtcg actatcttaa cggaattcta accgacttag
      901 aaggaaatat
//
