LOCUS       sequence-72385-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the signal peptide for 
            protein secretion of the Bacillus megaterium protein NprM under 
            control of the xylose inducible promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPNprM-hp
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(198..215)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
      181 aattcttgga ccggttagtg atggtgatgg tgatgcgatc ctctcaggcc ggcatgcggc
      241 cggtaccgga tcccgggagc tccggagatc ttcgaactag tcgcggcact gctcgtatgt
      301 gcaaaagcaa atgatgaaga aaggatacca actgataata atacctttaa agcctgtttt
      361 ttctttttca ttgtacattc acctccttga tttaagtgaa caagtttatc catcaactat
      421 cttaattgag ttagtttgtt tatccaataa actaacttta tctcatcata tattaattaa
      481 atgtttatgt caatgttttt tttagaaaat ttagttataa tattagatat gatactttta
      541 aatatctaat tcaagcttca aaaaacacca acttagttcg gtggataaac aaaggagtgg
      601 ttattattca aattgcagat caagctttag taaaaaaaat gaatcaaaaa ttaatattag
      661 atgaaatttt gaagaactcc cctgtctcca gggcaactct ctctgagatt acaggattaa
      721 acaagtctac tgtctcctct caagtaaata cactgcttga aaaagatttt atttttgaaa
      781 ttggggcagg gcaatctaga ggcggcagaa gacctgtaat gcttgttttt aataagaatg
      841 caggctactc gattggtatt gatataggag tcgactatct taacggaatt ctaaccgact
      901 tagaaggaaa tattattctc gaga
//
