LOCUS       sequence-72386-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence
            of the signal peptide for protein secretion of the Bacillus 
            megaterium protein YngK under control of the xylose inducible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPYngK+-hp
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(198..215)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
      181 aattcttgga ccggttagtg atggtgatgg tgatgcgatc ctctcaggcc ggcatgcggc
      241 cggtaccgga tcccgggagc tccggagatc ttcgaactag tatcagcttt agctggtaaa
      301 gcagaagaac agaataataa taagaataaa atagaaccaa tacatttttt aatatacatt
      361 gtacattcac ctccttgatt taagtgaaca agtttatcca tcaactatct taattgagtt
      421 agtttgttta tccaataaac taactttatc tcatcatata ttaattaaat gtttatgtca
      481 atgttttttt tagaaaattt agttataata ttagatatga tacttttaaa tatctaattc
      541 aagcttcaaa aaacaccaac ttagttcggt ggataaacaa aggagtggtt attattcaaa
      601 ttgcagatca agctttagta aaaaaaatga atcaaaaatt aatattagat gaaattttga
      661 agaactcccc tgtctccagg gcaactctct ctgagattac aggattaaac aagtctactg
      721 tctcctctca agtaaataca ctgcttgaaa aagattttat ttttgaaatt ggggcagggc
      781 aatctagagg cggcagaaga cctgtaatgc ttgtttttaa t
//
