LOCUS       sequence-72387-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal
            peptide for protein secretion using Bacillus megaterium under 
            control of the xylose inducible promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara
            E, Jahn D
  TITLE     High-yield intra- and extracellular protein production using 
            Bacillus megaterium.
  JOURNAL   Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 
            10.1128/AEM.00431-10. Epub 2010 Apr 30.
  PUBMED    20435764
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.00431-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-06"; volume: "76"; issue: "12"; pages: "4037-46"
COMMENT     Sequence Label: pSPAsp-hp
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(199..216)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
      121 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
      181 caattcttgg accggttagt gatggtgatg gtgatgcgat cctctcaggc cggcatgcgg
      241 ccggtaccgg atcccgggag ctccggagat cttcgaacta gtatcagcag atgctgttgt
      301 tggaagtaat gcacctaatg ctgctaaaag taatgctaca gctaatgctt tttttctttt
      361 acgcattgta cattcacctc cttgatttaa gtgaacaagt ttatccatca actatcttaa
      421 ttgagttagt ttgtttatcc aataaactaa ctttatctca tcatatatta attaaatgtt
      481 tatgtcaatg ttttttttag aaaatttagt tataatatta gatatgatac ttttaaatat
      541 ctaattcaag cttcaaaaaa caccaactta gttcggtgga taaacaaagg agtggttatt
      601 attcaaattg cagatcaagc tttagtaaaa aaaatgaatc aaaaattaat attagatgaa
      661 attttgaaga actcccctgt ctccagggca actctctctg agattacagg attaaacaag
      721 tctactgtct cctctcaagt aaatacactg cttgaaaaag attttatttt tgaaattggg
      781 gcagggcaat ctagaggcgg cagaagacct gtaatgcttg tttttaataa gaatgcaggc
      841 tactcgattg gtattgatat aggagtcgac tatcttaacg gaattcta
//
