LOCUS       sequence-72389-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Biedendieck R, Gamer M, Jaensch L, Meyer S, Rohde M, Deckwer WD, 
            Jahn D
  TITLE     A sucrose-inducible promoter system for the intra- and extracellular
            protein production in Bacillus megaterium.
  JOURNAL   J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
  PUBMED    17692983
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2007-12-1"; volume: "132(4)"; pages: "426-30. 
            Epub 2007 Jul 10"
COMMENT     Sequence Label: pRBBm69
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      232..820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     721..740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
       61 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      121 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      361 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      601 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg
      841 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
//
