LOCUS       sequence-72391-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the 
            phage K1E under control of the xylose inducible promoter PxylA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pK1E-RNAP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    345..366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(381..386,387..404,405..411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     424..446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     443..459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     443..459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      301 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      361 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      421 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
      481 tgcctgcagg tcatttatta acgattacct gcttttaatt ctgcatctaa ttgcataagc
      541 tccgccttca ttttttcctt ttcagcttca aaatcattat tcactggaag atcttctgct
      601 actgcatttt catcatttgt ttgtagccac tttggaacca tttctttacg tgcattacgt
      661 actacacgtt tcctttttgt ttgcttcggt ttccaatgtt taagacggta ttctaatagt
      721 gatttatatt gcttattcgt atcaacgatt acagtatcgt taagcatttc acttttcact
      781 tcgtcgaaag gtacattatt atatttttct cgtaatttat catcaataaa atcacgtgga
      841 ctac
//
