LOCUS       sequence-72392-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-SP6-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPSP6+t-gfp
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(319..339)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
      121 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat taattagctc
      181 caattcttgg accggcaaca aaaagaaccc tacctacgca aggtagatag ggcataggta
      241 accggttagc tagttagcat gcggccgcga attcattatt tgtagggctc atccatgcca
      301 tgtgtaatcc cagcagcagt tacaaactca agaaggacca tgtggtcacg cttttcgttg
      361 ggatctttcg aaagggcaga ttgtgtcgac aggtaatggt tgtctggtaa aaggacaggg
      421 ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgga tccatcttca
      481 atgttgtggc gaattttgaa gttagctttg attccattct tttgtttgtc tgccgtgatg
      541 tatacattgt gtgagttata gttgtactcg agtttgtgtc cgagaatgtt tccatcttct
      601 ttaaaatcaa taccttttaa ctcgatacga ttaacaaggg tatcaccttc aaacttgact
      661 tcagcacgcg tcttgtagtt cccgtcatct ttgaaagata tagtgcgttc ctgtacataa
      721 ccttcgggca tggcactctt gaaaaagtca tgccgtttca tatgatccgg ataacgggaa
      781 aagcattgaa caccataggt caaagtagtg acaagtgttg gccatggaac aggtagtttt
      841 ccagtagtgc aaataaattt aagggtaagc tttccgta
//
