LOCUS       sequence-72393-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-K1E-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-1+t-gfp
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(318..338)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt aattagctcc
      181 aattcttgga ccggcaacaa aaagaaccct acctacgcaa ggtagatagg gcataggtaa
      241 ccggttagct agttagcatg cggccgcgaa ttcattattt gtagggctca tccatgccat
      301 gtgtaatccc agcagcagtt acaaactcaa gaaggaccat gtggtcacgc ttttcgttgg
      361 gatctttcga aagggcagat tgtgtcgaca ggtaatggtt gtctggtaaa aggacagggc
      421 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacggat ccatcttcaa
      481 tgttgtggcg aattttgaag ttagctttga ttccattctt ttgtttgtct gccgtgatgt
      541 atacattgtg tgagttatag ttgtactcga gtttgtgtcc gagaatgttt ccatcttctt
      601 taaaatcaat accttttaac tcgatacgat taacaagggt atcaccttca aacttgactt
      661 cagcacgcgt cttgtagttc ccgtcatctt tgaaagatat agtgcgttcc tgtacataac
      721 cttcgggcat ggcactcttg aaaaagtcat gccgtttcat atgatccgga taacgggaaa
      781 agcattgaac accataggtc aaagtagtga caagtgttgg ccatggaaca ggtagttttc
      841 cagtagtgca aataaattta agggtaagct ttccgtatgt agcatcacct tcaccctctc
      901 cactgacaga aaatttgtgc ccattaacat caccatcta
//
