LOCUS       sequence-72394-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-K1E-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-2+t-gfp
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(317..337)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     932..960
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggcaacaaa aagaacccta cctacgcaag gtagataggg cataggtaac
      241 cggttagcta gttagcatgc ggccgcgaat tcattatttg tagggctcat ccatgccatg
      301 tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg
      361 atctttcgaa agggcagatt gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc
      421 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggatc catcttcaat
      481 gttgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta
      541 tacattgtgt gagttatagt tgtactcgag tttgtgtccg agaatgtttc catcttcttt
      601 aaaatcaata ccttttaact cgatacgatt aacaagggta tcaccttcaa acttgacttc
      661 agcacgcgtc ttgtagttcc cgtcatcttt gaaagatata gtgcgttcct gtacataacc
      721 ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatccggat aacgggaaaa
      781 gcattgaaca ccataggtca aagtagtgac aagtgttggc catggaacag gtagttttcc
      841 agtagtgcaa ataaatttaa gggtaagctt tccgtatgta gcatcacctt caccctctcc
      901 actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac
      961 tccagtgaaa agttct
//
