LOCUS       sequence-72396-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-K1E-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-1-gfp
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(238..951,952..954))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="ASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDEPYK"
     primer_bind     complement(281..301)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     896..924
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggtgaatcc gttcgcgagg tttagccggc atgcggccgc gaattcatta
      241 tttgtagggc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      301 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      361 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      421 tagttgaacg gatccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      481 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtact cgagtttgtg
      541 tccgagaatg tttccatctt ctttaaaatc aatacctttt aactcgatac gattaacaag
      601 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      661 tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      721 catatgatcc ggataacggg aaaagcattg aacaccatag gtcaaagtag tgacaagtgt
      781 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gctttccgta
      841 tgtagcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      901 taattcaaca agaattggga caactccagt gaaaagttct tctcctttgc tagcccaga
//
