LOCUS       sequence-72400-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter for RNA 
            polymerase of the phage K1E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-1
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             288..296
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(327..338)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggtgaatcc gttcgcgagg tttagccggc atgcggccgg taccggatcc
      241 cgggagctcc ggagatcttc gaactagttt ggaccattgt acatttcacc tccttataaa
      301 tcaaattgat aatttaggga gacatttctc ccttctatag tgtccagtaa atactgatct
      361 taaggaacgt acagacggct taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa
      421 ggtaaaggat aaaacagcac aattccaaga aaaacacgat ttagaaccta aaaagaacga
      481 atttgaacta actcataacc gagaggtaaa aaaagaacga agtcgagatc agggaatgag
      541 tttataaaat aaaaaaagca cctgaaaagg tgtctttttt tgatggtttt gaacttgttc
      601 tttcttatct tgatacatat agaaataacg tcatttttat tttagttgct gaaaggtgcg
      661 ttgaagtgtt ggtatgtatg tgttttaaag tattgaaaac ccttaaaatt ggttgcacag
      721 aaaaacccca tctgttaaag ttataagtga ccaaacaaat aactaaatag atgggggttt
      781 cttttaatat tatgtgtcct aatagtagca tttattcaga tgaaaaatca agggttttag
      841 tggacaagac aaaaagtgga aaagtgagac catggagaga aaagaaaatc gctaatgttg
      901 attactttga acttctgcat attcttgaat ttaaaaaggc tgaaagagta aaagat
//
