LOCUS       sequence-72402-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3)
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB62
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(310..330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(337..402)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggagtcgtcg accttggcca tctcgttgga gaagatctct tggaggtagc agatacggtt
       61 cttacgacgg gtgtaacgac gacgggcggt acgcttgaga cgggtggcct cggcggtctc
      121 tccggagtcg aagaggaggg ctccgatgag gttcttcttg atggagtgac ggtcggtgtt
      181 tccgaggacc ttgaacttct tggatgggac cttgtactcg tcggtgatga cggcccatcc
      241 gacggagttg gttccgatgt cgagtccgat ggagtacttc ttgtcggcgg ctgggactcc
      301 gtggattccg accttacgct tcttctttgg ggccatcttg tcgtcgtcgt ccttgtagtc
      361 gatgtcgtgg tccttgtagt ctccgtcgtg gtccttgtag tccatttttt tctagatgag
      421 caaagtgttt cccaactgaa aaaaaaacaa tttaatttaa aagtaagaag agtgcggacg
      481 gtagagagaa taaaagtcga aaaaattatc ctagaaactt ctaatatttt taaaaattaa
      541 aaaaatataa aaagtgaggt cacaaataag acagttagaa catttgttga aagcctaaaa
      601 taaaaattcg actaaacagt gagaacagga aaaacgctgg gactgaatgt ttgacctcat
      661 tataaattca taaaatttaa atttcctaat ctggataaaa acaaagataa aaacaaaata
      721 aaccaacaaa aaaccaacat gattagtcag atgaccagaa aactcacgtt aatttcacaa
      781 ggcccaaaaa aatctctccc cctctcgttg ctgcctgcac atct
//
