LOCUS       sequence-72405-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter for RNA 
            polymerase of the phage K1E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-2
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggtgaatcc gttcgcgagg tttagccggc atgcggccgg taccggatcc
      241 cgggagctcc ggagatcttc gaactagttt ggaccattgt acatttcacc tccttgtaag
      301 tgaaagttta atcaggcgct accgtagcaa cgccttgtta agccttctct tatagtgtca
      361 gctaaatagt ttgcttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
      421 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
      481 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
      541 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
      601 gttttgaact tgttctttct tatcttgata catatagaaa taacgtcatt tttattttag
      661 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
      721 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
      781 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
      841 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
      901 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
//
