LOCUS       sequence-72407-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter and a terminator 
            for RNA polymerase of the phage K1E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-1+t
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             324..332
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(363..374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggcaacaaa aagaacccta cctacgcaag gtagataggg cataggtaac
      241 cggttagcta gttagcatgc ggccggtacc ggatcccggg agctccggag atcttcgaac
      301 tagtttggac cattgtacat ttcacctcct tataaatcaa attgataatt tagggagaca
      361 tttctccctt ctatagtgtc cagtaaatac tgatcttaag gaacgtacag acggcttaaa
      421 agcctttaaa aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt
      481 ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga
      541 ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg
      601 aaaaggtgtc tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa
      661 ataacgtcat ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt
      721 ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat
      781 aagtgaccaa acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata
      841 gtagcattta ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag
      901 tgagaccatg gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc
      961 t
//
