LOCUS       sequence-72410-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter and a terminator 
            for RNA polymerase of the phage K1E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-2+t
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
      121 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt aattagctcc
      181 aattcttgga ccggcaacaa aaagaaccct acctacgcaa ggtagatagg gcataggtaa
      241 ccggttagct agttagcatg cggccggtac cggatcccgg gagctccgga gatcttcgaa
      301 ctagtttgga ccattgtaca tttcacctcc ttgtaagtga aagtttaatc aggcgctacc
      361 gtagcaacgc cttgttaagc cttctcttat agtgtcagct aaatagtttg cttaaggaac
      421 gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg gtttgtagac aaggtaaagg
      481 ataaaacagc acaattccaa gaaaaacacg atttagaacc taaaaagaac gaatttgaac
      541 taactcataa ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg agtttataaa
      601 ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt tctttcttat
      661 cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg cgttgaagtg
      721 ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac agaaaaaccc
      781 catctgttaa agttataagt gaccaaacaa ataactaaat agatgggggt ttcttttaat
      841 attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt agtggacaag
      901 acaaaaagtg gaaa
//
