LOCUS       sequence-72412-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg., encoding a promoter and a terminator 
            for RNA polymerase of the phage SP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPSP6+t
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(403..421)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(404..421)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
      121 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaatta attagctcca
      181 attcttggac cggcaacaaa aagaacccta cctacgcaag gtagataggg cataggtaac
      241 cggttagcta gttagcatgc ggccggtacc ggatcccggg agctccggag atcttcgaac
      301 tagtttggac cattgtacat ttcacctcct tttactatcc agccctcccc gtgaagactg
      361 gtgagtaata ggccgctcaa aagaacggca ctatacttct attctatagt gtcacctaaa
      421 tcgtatgctt aaggaacgta cagacggctt aaaagccttt aaaaacgttt ttaaggggtt
      481 tgtagacaag gtaaaggata aaacagcaca attccaagaa aaacacgatt tagaacctaa
      541 aaagaacgaa tttgaactaa ctcataaccg agaggtaaaa aaagaacgaa gtcgagatca
      601 gggaatgagt ttataaaata aaaaaagcac ctgaaaaggt gtcttttttt gatggttttg
      661 aacttgttct ttcttatctt gatacatata gaaataacgt catttttatt ttagttgctg
      721 aaaggtgcgt tgaagtgttg gtatgtatgt gttttaaagt attgaaaacc cttaaaattg
      781 gttgcacaga aaaaccccat ctgttaaagt tataagtgac caaacaaata actaaataga
      841 tgggggtttc ttttaatatt atgtgtccta atagtagcat ttattcagat gaaaaatcaa
      901 gggttttagt ggacaagaca aaaagtggaa aagtgagacc atg
//
