LOCUS       sequence-72413-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides GFP as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC014
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(51..53,54..56,57..767)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(96..117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(357..376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     704..725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(788..807)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(789..807)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(817..839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaggctcc atggtgagca
       61 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      241 ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      661 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      721 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtca gtgagaccga
      781 attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga
      841 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      901 taatagcgaa gagcc
//
