LOCUS       sequence-72414-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides mCherry as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC015
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             51..758
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(196..214)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     464..483
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(779..798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(808..830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaggctcc atggtgagca
       61 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      121 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      181 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      241 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      301 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      361 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      421 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      481 agacgatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
      541 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
      601 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
      661 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      721 gccgccactc caccggcggc atggacgagc tgtacaagtc agtgagaccg aattctcgcc
      781 ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      841 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      901 agagg
//
