LOCUS       sequence-72416-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides BFP as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC024
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(791..810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(792..810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(820..842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaggctcc atggtttcta
       61 agggtgaaga gcttatcaaa gagaatatgc acatgaagtt gtatatggag ggtactgtgg
      121 ataaccatca ctttaaatgt acatcagaag gagagggaaa gccttacgag ggtactcaaa
      181 caatgagaat taaggttgtg gagggtggtc ctcttccatt tgctttcgat atcttggcaa
      241 catcttttct ctacggttca aaaactttca ttaaccacac acaaggtatt cctgatttct
      301 ttaagcagtc ttttccagag ggattcactt gggaaagagt tactacttat gaggatggtg
      361 gtgttcttac tgcaactcaa gatacatctc ttcaggatgg atgcttgatc tataacgtta
      421 agatcagagg agttaatttc acttcaaacg gacctgttat gcaaaagaaa acattgggtt
      481 gggaggcttt tactgagaca ttgtacccag cagatggagg tttggaagga agaaacgata
      541 tggctcttaa attggttgga ggttctcatc tcatcgcaaa tattaaaact acatatagat
      601 caaagaaacc tgctaagaac ctcaaaatgc caggagttta ttacgtggat tacagacttg
      661 aaagaattaa agaggcaaac aacgaaactt atgttgaaca gcatgaggtt gctgtggcaa
      721 gatacgcagg actgggaggt ggacttaatg gtatggatga actttataag tcagtgagac
      781 cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg
      841 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      901 g
//
