LOCUS       sequence-72417-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3)
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB62
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(338..356)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(377..393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(377..393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(390..412)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    401..417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(425..431,432..449,450..455))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    470..491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(608..625)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaaattctc tcgctaattt atttgctttt ttaatgttat tattttatga ctttttatag
       61 tcactgaaaa gtttgcatct gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg
      121 tactttcaca atctctcttc aattccattt tgaagtgctt taaacccgaa aggttgagaa
      181 aaatgcgagc gctcaaatat ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa
      241 ctttattgtg ccgccaagaa aaaagtctca ttgcctaaga gaaacatatg tggttggaaa
      301 tcttccagat cgtggatcag agctccagct tttgttccct ttagtgaggg ttaatttcga
      361 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      421 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      481 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      541 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      601 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      661 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
//
