LOCUS       sequence-72424-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia with mCherry 
            reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-mCherry-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgccac catggttcca gagcccggcc ctgtcaacag
       61 tagcacccca gcctggggcc ccgggccacc gccagcccca gggggcagcg gctgggtggc
      121 cgctgcgctg tgcgtggtca tcgtgctgac ggcagccgcc aattcgctgc tgatcgcgct
      181 catctgcacg cagcccgcgc tgcgcaacac gtctaacttc ttcctggtgt cgctcttcac
      241 gtcggacctg atggtgggat tggtggtgat gcccccagcc atgctgaacg cgctgtatgg
      301 gcgctgggta ctagctcgcg gcctctgtct gctttggacc gccttcgacg tgatgtgctg
      361 cagcgcctcc attctcaacc tctgcctcat cagcctggac cgctacctgc tcatcctctc
      421 gccgctgcgc tacaagctgc gcatgacagc cccgcgtgcc ctggcgctca tcctgggtgc
      481 ctggagcctc gccgcgctgg cctccttcct gcccctcttg ctgggctggc atgaactggg
      541 caaagctcga acatctgccc cgggccagtg ccgcctattg gccagcctgc cttatgtcct
      601 cgtggcgtcc ggcgtcacct ttttcctgcc ttcgggtgcc atctgcttca cctactgcag
      661 gatcttgctg gctgcccgca agcaggcggt gcaagtggcc tcgctcacca cgggcacggc
      721 tacggctggc caggccttgg aaaccttgca ggtgcccagg acaccacgcc cagggatgga
      781 gtctgctgac agtaggcgtc tgaccaccaa gcatagcagg
//
