LOCUS       sequence-72429-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a default dummy as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD002
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(115..134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(116..134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(360..379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     479..501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(539..557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        625..729
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcatcaggt ggatcctaga
       61 taacctttac cttcattccc tgtaaattgg taccctgctg agaccgaatt ctcgccctat
      121 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      181 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      241 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      361 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      421 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      481 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      541 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      601 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      661 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      721 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      781 tgccttcctg tttttgctca cccagaaacg cttg
//
