LOCUS       sequence-72432-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the SV40 nuclear localization signal as GreenGate 
            C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD006
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(95..114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(96..114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(124..146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(340..359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     459..481
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(519..537)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        605..709
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcatcaggt cctaagaaga
       61 agaggaaggt ttgactgctg agaccgaatt ctcgccctat agtgagtcgt attacctggc
      121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      241 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      301 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
      361 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      421 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      481 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      541 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      601 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      661 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      721 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      781 cccagaaacg ctggtgaaag taaaagatgc tgaa
//
