LOCUS       sequence-72433-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides linker:nuclear localization signal as GreenGate C-terminal 
            tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD007
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(368..387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(369..387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(397..419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(613..632)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     732..754
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(792..810)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcatcaggt tctggttcag
       61 gttcaggttc tggatctgga agtggatctg gatcagcggc cgcatctgaa ttcaagcgtg
      121 aagagcaagc aaggaaagct aaggtgaaca atgagaaaaa gacggaaata gtgaaaccag
      181 agagttgtag caatgaagga gatgtcaagg atctgaaaag aaaggactct gaggatggaa
      241 acgagggtga ggaagaagaa gcttcttcga aaccgaaaaa gccaaaagtt gctctttctc
      301 atcttcagga cattgacgac acagaagctg atcaagaaga agagtaactg ctgagaccga
      361 attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga
      421 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      481 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      541 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
      601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      661 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      721 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      781 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      841 ttcttagacg tcaggtggca cttttcgggg aaat
//
