LOCUS       sequence-72434-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides an ER retrieval signal as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD008
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(86..105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(87..105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(115..137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(331..350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     450..472
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(510..528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        596..700
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(919..938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcatcaggt catgatgagc
       61 tttagctgct gagaccgaat tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt
      121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      181 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      241 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
      301 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
      361 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
      421 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
      481 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      961 gcaactcggt cgccgcatac actattctca gaa
//
